In a letter to Luton South MP Gavin Shuker, the retailer confirmed that the Luton branch will close on Saturday, May 4.
It is understood 77 jobs will be affected as a result of the closure.
Mr Shuker described the news as “disappointing” and said he had written to M&S to encourage them to find another store in Luton.
M&S stated: “We are continuing to consult on the people impact of the closure and colleagues will either be redeployed or offered redeployment to other M&S stores before redundancy is considered.
“We will have detailed discussions with colleagues in the coming weeks to determine what is best for their individual circumstances.”
Nearby M&S stores include:
> M&S Harpenden Foodhall (7 miles, 14 mins away)
> M&S St Albans (11.2 miles, 19 mins away)
> M&S Hemel Hempstead (12 miles, 18 mins away)